How to pull-out a specific PCR product?
Dr. Duncan Clark
duncan at genesys.demon.co.uk
Tue Feb 10 04:30:31 EST 1998
In article <34DE5EA3.88927433 at ouhsc.edu>, Kiley R. Prilliman <Kiley-
Prilliman at ouhsc.edu> writes
>Although I think it might depend on some factors (including the size of
>the product you want to pull out and what you want to do with it once
>you have it), you could always try biotinylated primers (and then
>separate the product using avidin-coated beads of some sort).
Any Tag would work but the problem is that all PCR products including
unwanted ones will also be labelled. What about PCR with normal primers
then PCR again using a tagged primer in a nested PCR.
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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