protein solubilizing out of SDS-PAGE gel

Stephen C. Dahl stebby at welchlink.welch.jhu.edu
Mon Feb 9 02:40:28 EST 1998


bipin k. dalmia (dalmiabk at phibred.com) wrote:
: hello:

: i am having trouble detecting a protein on SDS-PAGE gels. this is a
: very very soluble protein and i think that it may be solubilizing out
: of the gel during the staining steps. i am fixing the gel with
: 10%acetic acid/45% methanol and staining with CBB. is there any other
: way to ensure that the protein stays in the gel?


Can't say I've ever come across a protein *that* soluble, but if you think
this is the case, run the gel and immediately blot it onto .22
nitrocellulose and stain with amido black, india ink, ponceau or whatever
is your fancy.  Unless its a really small molecule you should see it
there.

Good luck,
Steve Dahl



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