Northerns using bacterial RNA
chriso at dfrc.wisc.edu
Tue Feb 10 10:36:30 EST 1998
In article <Pine.SUN.3.91.980206110245.29031A-100000 at ava.bcc.orst.edu>,
Mary Hagen <hagenm at bcc.orst.edu> wrote:
> Im interested in using nothern analysis to examine RNA from a Pseudomonas
> species. I haven't worked much with bacterial RNA and have a couple of
> basic questions:
> 1) Is bacterial RNA stable enough to anaylze satisfactorily using northerns?
> 2) What is commonly used as a control for quantitation of RNA?
> Also, if anyone has testimonials they would like to share regarding
> their personal experience working with bacterial RNA and northerns I
> would appreciate the information. Finally, any references regarding this
> type of work would be helpful.
> Thanks alot!
> Mary Hagen
I have recently finished a project of quantitating bacteria with slot
blots of RNA. I went the non-isotope route, and used Boehringer's
Digoxigenin system for detection. Email me, if you want the specifics.
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