Hi, Does anyone know how to efficiently separate very small (2.5kDa) proteins by PAGE? I'm trying the Tricine method now but can't get the very small markers (1.4 and 3.4kDa) to resolve, nor my tissue sample. Also does anyone know about the effect of electrophoresis on disulphide linkages will they be broken? Please contact me on r.patel at sheffield.ac.uk