polypeptide separation

H M Marriott R.Lawson at shef.ac.uk
Tue Feb 10 08:14:54 EST 1998


Hi,
Does anyone know how to efficiently separate very small (2.5kDa) proteins
by PAGE?  I'm trying the Tricine method now but can't get the very small 
markers (1.4 and 3.4kDa) to resolve, nor my tissue sample.
Also does anyone know about the effect of electrophoresis on disulphide 
linkages will they be broken?
Please contact me on r.patel at sheffield.ac.uk



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