an alternative to KW251 bacterial strain?

Mathieu Ingouff mathieu.ingouff at sgen.slu.se
Thu Feb 12 03:45:03 EST 1998


Dear netters,

We are planning to scrren a genomic library that was buried in our cold room=
=2E
I would like to know if it is really important to use KW251 bacterial
strain to propagate EMBL-T7-SP6 vector.

Can I use XL1-MRA instead? or another common strain?

KW251 phenotype: supE44, galK2, galT22, metB1, hsd R2, mcrB1, mcrA, (arg
A81::Tn10), recD1014

Thanks a lot!!

Mat



____________________________________
  Mathieu Ingouff, PhD student
  SLU, Department of  Forest Genetics
  Genetikv=E4gen 1B, Box 7027
  S-750 07 Uppsala, Sweden

  email: mathieu.ingouff at sgen.slu.se
  fax: +46.18.67.32.79.
 phone: +46.18.67.32.32.
____________________________________





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