Removing Bacteria from yeast cultures

Michael Lichten lichten at helix.nih.gov
Thu Feb 12 09:34:59 EST 1998


In article <6bukje$t6n at mserv1.dl.ac.uk>, wgschech at med.uni-tuebingen.de wrote:

> David,
> 
> By theory, pen/strep/amp should kill some bugs. If your yeasts are 
> really worth gold, you could try to grow diluted aliquots under 
> antibiotic restriction, but I'd rather throw them away.
> 
> Wolfi
> 
> 
> > Anyone tried methods other then amp/kan/tet for the removal of bugs
> > from yeast cultures? How about Pen/Strep or neomycin or gentamycin
> > even?! Any help would be appreciated. Dave

My neighbor once used chloramphenicol successfully to do just that.  Make
up some YPD plates with the drugs, and streak for single colonies.  Once
you have singles, streak 'em again.

-- 
Michael Lichten
lichten at helix.nih.gov
NIH
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