polypeptide separation

R Patel R.Patel at shef.ac.uk
Fri Feb 13 08:42:55 EST 1998


Hello All,
I'm trying to separate small (2.5kDa) proteins from human tissue.  I 
can't get the proteins to resolve on a tricine-gel, the sample appears 
to migrate a certain distance and then stop. Why?  I'm following the 
Schagger/Von Jagow method. Do I need a different SDS concentration?
HELP!

Please contact me on:-
r.patel at sheffield.ac.uk
 Thanks




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