detection of protein (20-30kd) by western

Didier Fesquet dfesquet at NS1.NIMR.MRC.AC.UK
Fri Feb 13 07:54:36 EST 1998

hi, I am having trouble to detect protein in the range of 20-30 kd by
western blotting. I am tranferring the gel onto Nitrocellulose(0.22um)
as well as immobilon P using semi dry tranfer (biorad apparratus) and
the recommended voltage 10volt , 30min for a mini gel. upon transfer, by
ponceau staining , I could not detect sharply stained protein band. what
any help is wellcome



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