polypeptide separation

R Patel R.Patel at shef.ac.uk
Fri Feb 13 09:18:55 EST 1998


Hello,
I'm trying to isolate a 2.5kDa protein by electrophoresis, from
human tissue.  I can't get the sample to resolve down the gel - the 
proteins appear to stop half way down the gel without further 
separation.
I'm using the Schagger/von Jagow method.
Shall I alter electrophoresis conditions,or SDS conc?
HELP

Please contact me on:-
R.Patel at sheffield.ac.uk




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