Hello, I'm trying to isolate a 2.5kDa protein by electrophoresis, from human tissue. I can't get the sample to resolve down the gel - the proteins appear to stop half way down the gel without further separation. I'm using the Schagger/von Jagow method. Shall I alter electrophoresis conditions,or SDS conc? HELP Please contact me on:- R.Patel at sheffield.ac.uk