polyT primer for PCR

Marc Champagne M.Champagne at cellbio.duke.edu
Sat Feb 14 14:27:39 EST 1998


>                                  Hello!
> 
> Does anybody have experience in using
> 
> polyT primers for PCR?
> 
> What Tm should I use, what are cycling conditions?
> 
> 
> polyT:  5' TTTTTTTTTTTTTTTTTTTTTTTC 3'
> 
> there are 23 dT and at the 3' end C, G or A
> 
> for this primer, Tm = 54,6
> 
> 
> Thanks!    Rimantas.Kodzius at sbg.ac.at

-----
In order to answer your question you need to tell us more about your PCR:

What's your template?

What do you expect your product will be?  A single specific product or a
library?

What's the other primer like?

m

-- 
Mon pays ce n'est pas un pays, c'est l'hiver (Vigneault).



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