polyT primer for PCR
M.Champagne at cellbio.duke.edu
Sat Feb 14 14:27:39 EST 1998
> Does anybody have experience in using
> polyT primers for PCR?
> What Tm should I use, what are cycling conditions?
> polyT: 5' TTTTTTTTTTTTTTTTTTTTTTTC 3'
> there are 23 dT and at the 3' end C, G or A
> for this primer, Tm = 54,6
> Thanks! Rimantas.Kodzius at sbg.ac.at
In order to answer your question you need to tell us more about your PCR:
What's your template?
What do you expect your product will be? A single specific product or a
What's the other primer like?
Mon pays ce n'est pas un pays, c'est l'hiver (Vigneault).
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