DNA concentration

Rick00100 rick00100 at aol.com
Mon Feb 16 14:48:32 EST 1998


Hi everyone,

I've been trying to gel purify my PCR products using the Qiagen kit.  After
purification I usually elute the DNA with 50ul water.  To do ligation, I'd like
to concentrate the DNA to about 6-8 ul.  When I EtOH ppt, I usually got bad
yield.  I heard that you can just speed-vac the DNA dissolved in water to
concentrate it.  Is that true?  What about DNA dissolved in buffers?

Thanks
Rick



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