[Q] HhaI vs CfoI, HpaII vs HapII
rsteinbe at etowah.ouhsc.edu
Mon Feb 16 17:46:20 EST 1998
yshan at tiamat.kaist.ac.kr wrote:
> Dear netters;
> HhaI and CfoI recognize and cut the same site, so do HpaII and HapII.
> But their sources are different. Is there any difference in activity between
> HhaI and CfoI, between HpaII and HapII? We have problem with HpaII digestion
> of genomic DNA. HpaII poorly cut unmethylated sites. I want to know if
> HapII is better than HpaII.
> Thanks in advance
> Yoonsoo Hahn
> __/ LIFE IS SHORT, DNA IS LONG \______________________________________________
> -. .-. .-. .-. .-. .-. . | Yoonsoo Hahn |
> ||X|||\ /|||X|||\ /|||X|||\ /| | Molecular Genetics Laboratory |
> |/ \|||X|||/ \|||X|||/ \|||X|| | Department of Biological Sciences, KAIST |
> ' `-' `-' `-' `-' `-' `- | yshan at bioneer.kaist.ac.kr (T) 042-869-5261 |
> ------------------------- http://bioneer.kaist.ac.kr/~yshan (P) 015-427-6466 |
I don't know about HapII, but there was a recent article in BioTechniques (Vol.
23: 843-847; Nov. 1997) suggesting that the problem with HpaII is that it likes to
bind to more than one site for good cutting. Using oligonucleotides with a HpaII
site, they could promote cutting of a viral unmethylated HpaII site.
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