inoue competent cells help !

Klaus Buchner klabuch at chemie.fu-berlin.de
Tue Feb 17 14:59:19 EST 1998



Robot-Boy wrote:

> hey all,
>
> just some quick help. I'm going to try making competent cells the inoue
> way, and only had the recipe for making the cells, but not for the actual
> transformation. Do I just do the transformation the "regular" way ?
> ie.thaw on ice, add DNA, wait 20-30 minutes, heatshock, chill, add SOC
> and grow at 37 deg for 60 minutes, plate on selective media.
>
> thanks in advance,
>
> Ed






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