Tru9 I vs Tru 509 I enzyme recog. site?

Anne Gillen ez049617 at catbert.ucdavis.edu
Thu Feb 19 20:48:07 EST 1998


Remember that DNA has a 5' and a  3' end and the strands are
antiparallel. The complement of 5'TTAA 3' is 5'TTAA 3' not 5'AATT 3'.

This is what the restriction site looks like:

5'TTAA 3'
3'AATT 5' 

 
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    Anne M. Gillen                           E-mail: amgillen at ucdavis.edu
    Pomology Department                      Phone:  (530) 752-1462
    University of California at Davis        New area code as of 11/1/97
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