Boyden Chamber-chemotaxis assay

Charlie Partridge crp2132 at pop.tamu.edu
Thu Feb 19 17:20:27 EST 1998


I am having trouble getting consistant results using a 48 well boyden
chamber.  It seems as though there bubbles forming in the lower chamber
after incubation (app. 5.5h), even though there are none present when I
put the membrane down.  If anyone knows how to remedy this or a good
technique paper, it would be greatly appreciated.
Thanks,
Charlie



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