dspinella at chugaibio.com
Fri Feb 20 11:54:25 EST 1998
> When the AUG start codon of the mRNA is embedded in the context of a
> good consensus Kozak sequence (e.g. RCCAUGG), the efficiency of ribosome
> binding (and thus translation) is enhanced. This is well established in
> prokaryotes where actual base-pairing between Kozak and ribosomal
> sequences occurs. In our hands with eukaryotic genes, protein
> expression following transfection is increased 2-3 fold when a poor
> Kozak sequence is replaced with the consensus shown above. So, while
> it's not absolutely essential to have a consensus Kozak sequence, it
> does help -- particularly with low-expresing constructs. Hope this is
> useful to you.
> --Dom Spinella
Oops. I should point out here that prokaryotes don't have an actual
Kozak sequence -- they have a Shine-Delgarno sequence which, although
located differently relative to start codons, plays a similar role.
Sorry -- sometimes my typing fingers are faster than my brain!
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