Genomic bacterial DNA PCR [Please re-mail answers]
Mr SCG Rison
srison at hgmp.mrc.ac.uk
Fri Feb 20 07:20:13 EST 1998
Thanks very much for the responses to my previous posting (copied below).
Unfortunately, I corrupted my mailbox and I have lost all answers before
I was able to hard-copy them.
I would be extremely grateful if people who got in contact with me (both
to provide answers and to ask about replies I received) could re-mail me.
I will post a summary of answers to the newsgroup.
Thanks in advance,
- Original message follows -
Has anybody got any protocols/suggestions on how to perform PCR with a genomic
(E. coli) bacterial DNA template. Is there a direct way or do I first have to
isolate genomic DNA and then PCR (and if so, what protocol(s) do you suggest?).
Could you please e-mail me answer at: stuart at ludwig.ucl.ac.uk as well as post
Ludwig Institute for Cancer Research (UCL)
Tel. (0171) 878 4127 Fax. (0171) 878 4040 e-mail: stuart at ludwig.ucl.ac.uk
- End of original message -
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