Solubilize peptide

ISOAI Atsushi aisoai at
Mon Feb 23 09:17:20 EST 1998

In article <l03102804b116fcd188ab@[]>, 
jaikawa at UCSD.EDU says...
>If the peptide (17 a.a.) is hard to be dissoloved in water-based buffer,
>what solvent(and how much) should be added? Give me any 

I'd like to recommend you to add acetic acid, trifluoro acetic
acid (TFA), and acetonitrile. How much ?  Case by case,
but, in the case of acetic acid or TFA, try 0.1% to 10%. In AcCN,
5% to 4,50%. 

ISOAI Atsushi, Ph.D. <aisoai at>
Research Center, Asahi Glass Co.LTD.

More information about the Methods mailing list