Solubilize peptide

ISOAI Atsushi aisoai at agc.co.jp
Mon Feb 23 09:17:20 EST 1998


In article <l03102804b116fcd188ab@[132.239.200.143]>, 
jaikawa at UCSD.EDU says...
>
>If the peptide (17 a.a.) is hard to be dissoloved in water-based buffer,
>what solvent(and how much) should be added? Give me any 
suggestions.


I'd like to recommend you to add acetic acid, trifluoro acetic
acid (TFA), and acetonitrile. How much ?  Case by case,
but, in the case of acetic acid or TFA, try 0.1% to 10%. In AcCN,
5% to 4,50%. 

-- 
ISOAI Atsushi, Ph.D. <aisoai at agc.co.jp>
Research Center, Asahi Glass Co.LTD.
http://www.yk.rim.or.jp/%7Eaisoai/molbio-j.html




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