his-tag

[Souren Mkrtchian]

On 13 Feb 1998 13:31:55 -0800, hroychow at NMSU.EDU (Hiranya
Roychowdhury) wrote:

>Colleagues,
>        Need some wisdom! Would like to hear from those that have used the
>histidine tags in protein expression (the his-tag/Ni column stuff). Between
>the Pharmacia's and Qiagen's, which one works better in terms of
>reproducibilty and yield. What are the +/- 's in them?
>
>Thank you in advance.
>
>Hiranya.


We tried both and Qiagen turned to be much better in all respects.


Regards,

Souren Mkrtchian