his-tag

Souren Mkrtchian soumkr at ki.se
Mon Feb 23 08:25:51 EST 1998


On 13 Feb 1998 13:31:55 -0800, hroychow at NMSU.EDU (Hiranya
Roychowdhury) wrote:

>Colleagues,
>        Need some wisdom! Would like to hear from those that have used the
>histidine tags in protein expression (the his-tag/Ni column stuff). Between
>the Pharmacia's and Qiagen's, which one works better in terms of
>reproducibilty and yield. What are the +/- 's in them?
>
>Thank you in advance.
>
>Hiranya.


We tried both and Qiagen turned to be much better in all respects.


Regards,

Souren Mkrtchian





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