Kanamycin selection

Jun-Ichi Aikawa jaikawa at UCSD.EDU
Tue Feb 24 18:22:28 EST 1998


What concentration of kanamycin is used for the section of recombinant E.
coli? I bothered high background of non-recombinant clones. My conditions
are as follows:
kanamycin: 30 ug/ml in LB plate (according to Current Protocol)
E. coli: XL2-Blue MRF, XL1-Blue, DH5a (commercial competent cell)
plasmid: pBK-CMV, pEGFP-N1

Please give me any suggestion and comments.

Jun-ichi Aikawa, Ph.D.
University of California, San Diego





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