Hepl with synthesis of long cDNAs

Erik Mottl mottlec at IASTATE.EDU
Wed Feb 25 14:20:40 EST 1998


Does anyone know of a good method to synthesize long cDNAs?  We are =
working with plant tissues and using a combination of RNA purification =
and cDNA synthesis kits that tends to generates average insert sizes of =
about 600 bp.   We would prefer the average insert size to be around 1.5 =
kb.




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