annealing of GC rich oligonucleotides

G. Dellaire gdellaire at PO-BOX.MCGILL.CA
Thu Feb 26 12:05:02 EST 1998


Hello,
I'm trying to anneal two GC-rich oligonucleotides (86% G+C, 41 bp). Does
anyone know of a way to improve this annealing (other than only
temperature changes) - for example, is there a protein (SSB, recA, gene
32 protein...), or a buffer or a product which facilitates the process? 

Thanks very much for your help


Nathalie Savard



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