problems with reverse primer

Mag. Dorian Winter dorian.winter at akh-wien.ac.at
Thu Feb 26 04:25:23 EST 1998


Here´s one for you PCR-gods out there....

I want to amplify a cDNA sequence of about 1000 bp.
The forward primer is
5´-AAGCTTATGGCCATGCTCCTGGGG-3´
The reverse primer is
5´-GCCGACACCAGGTCGACTAAGCTT-3´

I don´t get any product . The forward primer works ok as checked with
other, internal primers, but I can´t get the reverse primer to work.
I can´t change the location very much, as it contains a stop codon I
need.

I would be grateful for any brilliant idea what might be the matter or
how I could solve the problem.

Dorian Winter



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