UV crosslinking

HK hakorsmo at facstaff.wisc.edu
Thu Feb 26 10:11:14 EST 1998

	We are studying a nuclear protein which binds to a promotor element. We have 
identified the protein by Western analysis as a previously described homeodomain protein.  
Our problem is to explain the fact that though on Western analysis the protein is 38 kDa,  when 
the protein in a nuclear extract is UV crosslinked to the appropriate BrdU-substituted oligo, 
SDS-PAGE suggests a 60 kDa protein.
	Any thoughts on an explanation for this? Crosslinking of both our protein and an 
unrelated protein to the DNA seems most likely to us.

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