Long distance (RT) PCR
deborah_hailstones_at_emai at smtpgwy.agric.nsw.gov.au
Thu Feb 26 21:03:15 EST 1998
We are planning to use RT-PCR to amplify long sections of a plant
virus, hopefully up to 9.5 kb... it seems quite likely we will need
some propritary kit to achieve this, does anyone have any information/
direct experiences to share on kits for such long reactions (for
either RT-PCR or PCR)? How about home-made mix-and-match set ups?
Also oligo quality (eg HPLC grade vs regular etc)?
More information about the Methods