Long distance (RT) PCR

Deborah Hailstones deborah_hailstones_at_emai at smtpgwy.agric.nsw.gov.au
Thu Feb 26 21:03:15 EST 1998


     G'day...
     
     We are planning to use RT-PCR to amplify long sections of a plant 
     virus, hopefully up to 9.5 kb...  it seems quite likely we will need 
     some propritary kit to achieve this, does anyone have any information/ 
     direct experiences to share on kits for such long reactions (for 
     either RT-PCR or PCR)?  How about home-made mix-and-match set ups? 
     Also oligo quality (eg HPLC grade vs regular etc)?
     
     MTIA
     
     Deb





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