Kanamycin selection

[SHENGYANG]

jaikawa at ucsd.edu (Jun-Ichi Aikawa) wrote:
 >What concentration of kanamycin is used for the section of recombinant E.
>coli? I bothered high background of non-recombinant clones. My conditions
>are as follows:
>kanamycin: 30 ug/ml in LB plate (according to Current Protocol)
>E. coli: XL2-Blue MRF, XL1-Blue, DH5a (commercial competent cell)
>plasmid: pBK-CMV, pEGFP-N1
>
>Please give me any suggestion and comments.
     We use 10ug/ml in LB plate and no high background has been watched.
I think it might not be caused by kanamycin concentraction but the clone
scheme.

Yang Sheng
Shanghai Inst. of Biochem.
Tel: 86-21-64374430-243

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