Bcl digestion

Ramaswamy K. Iyer riyer at pathology.medsch.ucla.edu
Thu Feb 26 13:39:49 EST 1998


Hi Hua,

BclI is sensitive to dam methylation.  Grow the plasmid in a dam- strain
such as GM2163 or JM110, extract DNA and then it will cut beautifully
with BclI.  

Hope this helps.

Ram Iyer
riyer at pathology.medsch.ucla.edu
riyer at ucla.edu



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