Antibody Purification: better method than western technique?

S. Findley polliwog at
Fri Feb 27 05:08:58 EST 1998

I have serum from an immunized bunny.
Is there a more gentle or efficient method for the purification of
antigen-specific antibodies from total serum than the technique
in which you western blot your antigen and affinity purify the specific
antibodies folloed by a nasty pH elution?

-any suggestions appreciated!


Seth D. Findley               polliwog at
Department of Biochemistry    Phone: 206-543-1710
Health Sciences Building J579
University of Washington, Box 357350, Seattle, WA 98195

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