His6-Tagged Protein: Binding strength, panning...

S. Findley polliwog at u.washington.edu
Fri Feb 27 04:56:45 EST 1998


Ok I have a his6-tagged protein which I would like to immobilize on a
Ni-NTA column and subsequently bind and purify interacting proteins by
passing a cell extract over the column.

Is this a generally used approach?
Are there preferable tags to immobilize proteins on columns?
(Is th affinity of His6 for the Ni sufficient for this type of protocol?


Thanks


SDF
-- 
Seth D. Findley               polliwog at u.washington.edu
Department of Biochemistry    Phone: 206-543-1710
Health Sciences Building J579
University of Washington, Box 357350, Seattle, WA 98195



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