His6-Tagged Protein: Binding strength, panning...

S. Findley polliwog at u.washington.edu
Fri Feb 27 04:56:45 EST 1998

Ok I have a his6-tagged protein which I would like to immobilize on a
Ni-NTA column and subsequently bind and purify interacting proteins by
passing a cell extract over the column.

Is this a generally used approach?
Are there preferable tags to immobilize proteins on columns?
(Is th affinity of His6 for the Ni sufficient for this type of protocol?


Seth D. Findley               polliwog at u.washington.edu
Department of Biochemistry    Phone: 206-543-1710
Health Sciences Building J579
University of Washington, Box 357350, Seattle, WA 98195

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