cloning NdeI cut products

Richard P. Grant spam.blocked at
Fri Feb 27 04:05:04 EST 1998

Amy Anne Caudy wrote:
> You'll need to use quite a bit to
> see it on EtBr, and of course the fragment size has to be separable. 

Which reminds me.  Viewing the gel under short wave UV (i.e. a
transilluminator) can cause dT dimers which the bugs will spit out,
significantly reducing the (apparent) cloning efficiency.  You should always
use a long wave (therefore lower energy) source of UV, e.g. a hand-held lamp.


Richard P. Grant MA DPhil  | Cambridge Molecular Technologies
Senior R&D Scientist       | rgrant at cmtech co uk
Tel: +44 1223 508345       |
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