cloning NdeI cut products
Richard P. Grant
spam.blocked at delete.this.cmtech.co.uk
Fri Feb 27 04:05:04 EST 1998
Amy Anne Caudy wrote:
> You'll need to use quite a bit to
> see it on EtBr, and of course the fragment size has to be separable.
Which reminds me. Viewing the gel under short wave UV (i.e. a
transilluminator) can cause dT dimers which the bugs will spit out,
significantly reducing the (apparent) cloning efficiency. You should always
use a long wave (therefore lower energy) source of UV, e.g. a hand-held lamp.
Richard P. Grant MA DPhil | Cambridge Molecular Technologies
Senior R&D Scientist | rgrant at cmtech co uk
Tel: +44 1223 508345 | http://www.cmtech.co.uk/
-- Standard corporate disclaimers apply --
More information about the Methods