Methods February 1998 Archives by author

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Starting: Sun Feb 1 10:07:24 EST 1998
Ending: Sat Feb 28 23:02:07 EST 1998
Messages: 727

• address for Exgen 500   최웅
• GFP Vector   A.Habel
• BIOSCI/bionet miniFAQ & Fundraiser   BIOSCI Administrator
• RNA from plant roots   Tanvir Ahmad
• Solubilize peptide   Jun-Ichi Aikawa
• Solubilize peptide   Jun-Ichi Aikawa
• Kanamycin selection   Jun-Ichi Aikawa
• need BL21A(DE3) strain of E. coli   Ashok Aiyar
• Dam methylation and BclI   Ashok Aiyar
• E.coli genome on a chip?   David Alexander
• DNA isolation from hair   Michael Allen
• DNA isolation from hair   Michael Allen
• detection of protein (20-30kd) by western   Michael Allen
• Polypropylene Racks for Eppendorf tubes   Michael Allen
• Deoxycoformicin source   Michael Allen
• Test (please ignore)...   Asteras Amaliadas
• Solubilisation of GST-fusion proteins?   Asteras Amaliadas
• Rare arg codon causing expression problems?   Chen Ho An
• cloning NdeI cut products   John S Jacobs Anderson
• cloning NdeI cut products   John S Jacobs Anderson
• cloning NdeI cut products   John S Jacobs Anderson
• RNase Protection Assay; non-specific?   Rolands G. Aravindan
• RNase Protection Assay; DEPC for everything?   Rolands G. Aravindan
• RNase Protection Assay; non-specific?   Rolands G. Aravindan
• photographing invertebrates   Michael Ashenbach
• Solubilize peptide   ISOAI Atsushi
• Solubilize peptide   ISOAI Atsushi
• MC1061/P3??   Shane Atwell
• nuclear extracts   Joel Baguet
• UV crosslinking   Joel Baguet
• semi-specific PCR   Dr. Andrew Bahn
• semi-specific PCR   Dr. Andrew Bahn
• semi-specific PCR   Dr. Andrew Bahn
• Acrylamide Polymerization   Bailey1159
• test   Greg Baker
• Polyclonal Antibody Questions   Greg Baker
• Advice on sterilizing transfer pipets   Bambey
• Postdoctoral Research Fellow   Tim Barnett
• Kanamycin selection   Sharon Barr
• contaminated Taq   Joseph Michael Bay
• Killer gene/protein?   Joseph Michael Bay
• Experiences with Clontech Genome Walker Kits   Clifford Bellone
• [Q] ISCO electro-eluter ( the white one)   Claude Besmond
• Enriched library for Microsatellites   Dinakar Bhattramaki
• ? Ethidium Bromide Protection?   Bike&Linux
• soutern problems   Bike&Linux
• Commercially available cut vectors   Stephen Billington
• Western for large membrane protein   Shaun D. Black
• PLEASE Help: Wheat genome size?????   N.W. Blackhall
• Seeking Arabidopsis 2hybrid library   Frederik Boernke
• Q:Pichia kit   Frederik Boernke
• EtBr and heat denatured DNA   Frederik Boernke
• lactose instead of IPTG?   Frederik Boernke
• Throw gloves after handling EtBr?   Frederik Boernke
• Yeast 2 hyb screening   Frederik Boernke
• Throw gloves after handling EtBr?   Wayne Bolton
• Hot Start Wax Beads   Wayne Bolton
• PCR machines   Cres Bookstein
• fungal dna extraction   Jim Brannigan
• PCR machines   Lisa J Brunet
• tets   Bubby
• Throw gloves after handling EtBr?   Bubby
• inoue competent cells help !   Klaus Buchner
• kozak's sequence   Sebastian Bunka
• Throw gloves after handling EtBr?   Sebastian Bunka
• Kanamycin selection   Sebastian Bunka
• Plasmid(s) needed   Sebastian Bunka
• Autoclaved MOPS: is it supposed to be yellow?   Brett Burkholder
• philadelphia chromosome diagnostics   Thomas Burmeister
• Pyrococcus woesei   C.L.L.
• Pyrococcus woesei   C.L.L.
• Looking for tetO-lacZ plasmid   Sidney Cambridge
• tomato and cucumber DNA libraries   James J. Campanella
• Lymphoma cDNA library   Debbie Carter
• Best transfection method for CHO cells   Ed Castro
• cloning NdeI cut products   Amy Anne Caudy
• polyT primer for PCR   Marc Champagne
• Human cDNA clone of Lck   Alex Chang
• test message please ignore   Jon Chappel
• Throw gloves after handling EtBr?   Tom Chappell
• Uneven PCR, Rapid method to walk unknown sequence, http://128.84.203.244   Xiongfong Chen
• Rat Actin Probe   Robert Yuk Sing Cheng
• Isolation advice   Joanne & Christopher
• Tris-Tricine Gel?   Namjin Chung
• Good general bioinformatics reference   Darryl L. Clark
• Throw gloves after handling EtBr?   Darryl L. Clark
• annealing of GC rich oligonucleotides   Darryl L. Clark
• How to pull-out a specific PCR product?   Dr. Duncan Clark
• What is the highest DNA concentration in a buffer   Dr. Duncan Clark
• ATCC plasmids in EU?   Dr. Duncan Clark
• Bcl digestion   Dr. Duncan Clark
• PCR with degenerate primer   Dr. J.P. Clewley
• bionet.molbio.methds-reagnts Msg # 56500   Jean-Marc Collard
• GST fusion protein cleavage problem   Seth Corey
• I thought you might be interested.   CoryA at INAME.COM
• SSCP kits   Marc Crepeau
• DNA concentration   Marc Crepeau
• Homemade ECL Plus reagents?   Sean Cutler
• request reagent   DOCTORHIM
• EDTA/Heparin & DNA stability   DOCTORHIM at AOL.COM
• Automatic Sequencing service wanted   DOCTORHIM at AOL.COM
• Contaminated -Taq   DOCTORHIM at AOL.COM
• High molecular weight marker   DOCTORHIM at AOL.COM
• lactose instead of IPTG?   DOCTORHIM at AOL.COM
• protein solubilizing out of SDS-PAGE gel   Stephen C. Dahl
• bleaching GFP   Nico Dantuma
• Cell cycle reagents for avian cells   Rana Datta
• RNase Protection Assay; non-specific?   David L. Haviland, Ph.D.
• Help! Incredible high immunfluorescence background!   David L. Haviland, Ph.D.
• RNase Protection Assay; DEPC for everything?   David L. Haviland, Ph.D.
• RNase Protection Assay; non-specific?   David L. Haviland, Ph.D.
• Site-directed mutagenesis   David L. Haviland, PhD
• Autoclaved MOPS: is it supposed to be yellow?   David L. Haviland, PhD
• DTT and BME   Greg Davis
• 5-Methyl dNTP Source?   Rick Davis
• DNA quantitation : what a pain   Dean.Sequera at am.apbiotech.com
• annealing of GC rich oligonucleotides   G. Dellaire
• Antibody to any mitochondrial protein   Mohanish Deshmukh
• Help: Modified Western.   John T. Dimos
• Difficulty with expression of 160 Kda monomeric protein in E. coli   Benan Dincturk
• Detection of Prokaryotic terminators   Benan Dincturk
• How do you calculate the molecular weight of the protein using GCG?   Benan Dincturk
• DNA concentration   Benan Dincturk
• cloning large PCR bits   Benan Dincturk
• Spinach genomic library or root cDNA library   Benan Dincturk
• BioRad Gene Cycler   Benan Dincturk
• kozak's sequence   Benan Dincturk
• pGEX vectors   Benan Dincturk
• colony hybridization   Benan Dincturk
• GFP Vector   Dr Andrew Doherty
• Western for large membrane protein   Dr Andrew Doherty
• End labelling oligos for screening northerns?   Harold J. Drabkin
• Ubiquity of cDNA Sequences???   Carla Drebing
• Hot Start Wax Beads   Richard J. Dudley
• Kanamycin selection   Richard J. Dudley
• Expression cloning using xenopus oocytes   Donald Dunbar
• Oxygene entry into cells   Francis Durst
• How to transfer DNA in polyacrylamide gel to Nylon(N+) filters   ELADER at AMBION.COM
• Northern blot re-probing   ELADER at AMBION.COM
• PCR machines   Dr. Klaus Eimert
• How do you calculate the molecular weight of the protein using GCG?   Roger Fachini
• removal of hexahistidine tag by peptidase   Frank O. Fackelmayer
• LITMUS 18 vector: Is there a XmnI site in it?   Frank O. Fackelmayer
• Help: Modified Western.   Frank O. Fackelmayer
• detection of protein (20-30kd) by western   Didier Fesquet
• His6-Tagged Protein: Binding strength, panning...   S. Findley
• Brent/Clontech yeast 2-hybrid screen: recombination/rearrangements.   S. Findley
• Antibody Purification: better method than western technique?   S. Findley
• LINE inserts.   Karl Fischer
• nucleoside analogues: pH/conc.to dissolve??   Karl Fischer
• Commercially available cut vectors   Karl Fischer
• CAT 3MS and pSP72   Karl Fischer
• LINE inserts.   Bryan L. Ford
• Throw gloves after handling EtBr?   Bryan L. Ford
• semi-specific PCR   Bryan L. Ford
• semi-specific PCR   Bryan L. Ford
• Anti-actin antibody problem - Sigma   Bart Frank
• U.V Crosslinking   Iddo Friedberg
• biotinylated cloning   Roderic Fuerst
• No subject   GENYAN
• How to transfer DNA in polyacrylamide gel to Nylon(N+) filters   GENYAN
• How to extract DNA from polyacrylamide gel   GENYAN
• genomic sourthern, help needed   GENYAN
• genomic sourthern, help needed   GENYAN
• About PCR   GENYAN
• about pcr   GENYAN
• Glycoproteins   Vijay Gadkar
• in situ conundrum   Daniela Gadotti
• in situ newsgroup?   Daniela Gadotti
• thiostrepton resistance   Jorge L. Galazzo
• What is SAGE?   Olivier Gandrillon
• Cell cycle reagents for avian cells   Olivier Gandrillon
• how to keep stable transfections?   Mike Garbutt
• cloning NdeI cut products   Mike Gardner
• Contaminated -Taq   Denis Gaucher
• fluorescence quenching   Mr. J Gaughan
• 15-mer binding to membrane. Is it possible?   Dr. Peter Gegenheimer
• Problems with S1 digestion   Dr. Peter Gegenheimer
• normalizing northerns of plants   Dr. Peter Gegenheimer
• Sequensing directly on the genom   Yoram Gerchman
• How to pull-out a specific PCR product?   Yoram Gerchman
• How to pull-out a specific PCR product?   Yoram Gerchman
• Genomic bacterial DNA PCR.   Gerd
• colony hybridization   Gerd
• Yeast 2 hyb screening   Mohammad H Ghahremani
• Tru9 I vs Tru 509 I enzyme recog. site?   Anne Gillen
• Pyrogen Free/Low LPS Bacterial Strains   Dave Gilliss
• Contaminated -Taq   Michelle Gleeson
• Polypropylene Racks for Eppendorf tubes   Michelle Gleeson
• Polypropylene Racks for Eppendorf tubes   Tom Glenn
• Mouse Library   Stephane Gobron
• Trying to find company/university to develop enzyme assays   Steven Goldberg
• Rare arg codon causing expression problems?   Steven Goldberg
• ECL direct system   Steven Goldberg
• need BL21A(DE3) strain of E. coli   Daniel Gonzalez
• CpG methylation   J.S Good
• DNA quantitation : what a pain   J.S Good
• Better chelator of zinc than EDTA?   Damaged Goods
• Contaminated -Taq   David de Graaf
• RDA-(Rep. Diff. Analy.) problems and solutions?   Jim Graham
• Clontech "Suppression" Subtraction -Excuse Me?   Jim Graham
• Clontech "Suppression" Subtraction -Excuse Me?   Jim Graham
• cDNA precipitation   Richard P. Grant
• contaminated Taq   Richard P. Grant
• competant cells   Richard P. Grant
• cloning NdeI cut products   Richard P. Grant
• contaminated Taq   Richard P. Grant
• cloning NdeI cut products   Richard P. Grant
• contaminated Taq   Richard P. Grant
• High pH buffer?   Richard P. Grant
• cloning NdeI cut products   Richard P. Grant
• Ubiquitous primers   Richard P. Grant
• Automated nucleic acid extraction   Dr. J. Green
• Heteroduplex mobility assays   Dr. J. Green
• Contaminated-Taq   Paul C. Grimm
• LINE inserts.   Joel Guss
• LINE inserts.   Joel Guss
• LINE inserts.   Joel Guss
• Throw gloves after handling EtBr?   John H.
• UV crosslinking   HK
• Northerns using bacterial RNA   Mary Hagen
• Plasmid(s) needed   R.D. Haigh
• Long distance (RT) PCR   Deborah Hailstones
• Hot Start Wax Beads   Alastair Hamilton
• Contaminated -Taq   Robert G. Hamilton
• contaminated Taq   Robert G. Hamilton
• detecting His-tagged gene products   Theo Hanekamp
• Q:Pichia kit   Dimitri Harmegnies
• Pichia kit vs Yeast Amino-Terminal FLAG Expression Kit   Dimitri Harmegnies
• End labelling oligos for screening northerns?   Elizabeth Harrison
• High molecular weight marker   Randy Haun
• CREB AB   Randy Haun
• Epicurian Coli XL1-Red cells   Robin Haw
• Host & Preparation of pJM17 or pBHG10   Scott Henderson
• Help - Outline for Methods Course   Paul N. Hengen
• contaminated Taq   Herz-Kreislauf-Physiologie
• pYAC55 (Not I) availability   David Hills
• pYAC55 Please?   David Hills
• Removing Bacteria from yeast cultures   David Hills
• How to prevent S35 contamination in tissue culture?   David Hills
• How to extract DNA from polyacrylamide gel   Dr. Robert Hindges
• emsa   Miss. V.M. Hodges
• literature for rna secondary structure?   Kerstin Hoef-Emden
• Making RNA probe   Simon Hoffenberg
• GFP antibody for immunolocalization   Daniel Hofius
• ATCC plasmids in EU?   Carsten Hohoff
• lactose instead of IPTG?   Carsten Hohoff
• How to pull-out a specific PCR product?   Annette C. Hollmann
• Lipid removal pre-SDS-PAGE   Louis Hom
• Protein expression in pTrc99A, Please help !!   Preecha Homchampa
• [Fwd: Re: Spooling coli DNA]   Toby M Horn
• Chelex reagent!   Ian & Viive Howell
• N-Term 6-His Eukaryotic Expr. Vector?   Charles Hubbard
• Clontech, it' not a problem !!   Jürgen Häußler
• Contaminated -Taq   Iladol
• an alternative to KW251 bacterial strain?   Mathieu Ingouff
• Western for large membrane protein   Wendy Ingram
• Tatgene/HIV-1LTR reporter   Ernest Isabelle
• tatgene/HIV-1 LTRreportergene   Ernest Isabelle
• HygB resistance of E.coli   Ramaswamy K. Iyer
• Bcl digestion   Ramaswamy K. Iyer
• Hygromycin selection of E. coli   Ramaswamy K. Iyer
• GFP Vector   Fraser Dr N J
• How much total RNA ?   Fraser Dr N J
• RT-PCR from incredibly small amounts of tissue   Fraser Dr N J
• cDNA library screening probs.   VASANTHARAJAN JANAKIRAMAN
• oligomerization   JOSE LUIS CARRASCO JIMENEZ
• oligomerization   JOSE LUIS CARRASCO JIMENEZ
• protein x Freund's adjuvant   ITABAJARA DA SILVA VAZ JUNIOR
• ECL direct system   Jaana
• streptavidin columns?   Jack_Leonard at millipore.com
• packaging protocol   Christophe Jacob
• cDNA precipitation   Christophe Jacob
• Liposomes   James
• Unsilanizing sequencing plates?   Jason A. Jarzembowski
• Electrotransfer of high MW proteins   Csaba Jeney
• kozak's sequence   Ju-Hong Jeon
• Construction of genomic library.   Sally N. Jewell
• M -- please check my calculation   John
• competant cells   Iain Johnstone
• primers for dog P450   Raija Jonsson
• inoue competent cells help !   Zophonias O. Jonsson
• contaminated Taq   Zophonias O. Jonsson
• RNA extraction kits   Juck, David
• Automatic Sequencing service wanted   K.Scott at botany.uq.edu.au
• address for Exgen 500   Alan KAY
• EtBr and heat denatured DNA   Alan KAY
• Flouro OMP source?   Chris Kafer
• Antibody against lambda-phage   Liselote Kahns
• Antibody against lambda-phage   Liselote Kahns
• Free Power Supply   Susan Kang
• Free Power Supply   Susan Kang
• inoue competent cells help !   Susan Kang
• Pulse power supply   Susan Kang
• RNase Protection Assay; DEPC for everything?   Fumiki Katsuoka
• RNA extraction from blood   T. Jeffrey Keen
• PCR with degenerate primer   Jill Kellogg
• How to pull-out a specific PCR product?   Jill Kellogg
• PLEASE Help: Wheat genome size?????   Gabre Kemp
• Primers for p53 and K-ras   Christiane Kim
• [Que] Electroporation   Hyunmin Kim
• EtBr and heat denatured DNA   Rob Kirkpatrick
• Western specicity cross contamination   Nameer B Kirma
• Chelex reagent!   Csaba Kiss
• Help:Schistosoma japonicum GST   Jongrak Kittiworakarn
• ? Ethidium Bromide Protection?   Jongrak Kittiworakarn
• Post-doc position/Sweden (plant mol. biol.)   Leszek A. Kleczkowski
• polyT primer for PCR   Rimantas Kodzius
• polyT for PCR   Rimantas Kodzius
• HPLC-Manager-Software   Lars Komorowski
• E. coli signal sequences   Lars Komorowski
• Clontech's Talon   Lars Komorowski
• E.coli genome on a chip?   Paul Kowalski
• NEB "Improved" IMPACT T7 expression system   Joel A. Kreps
• UV crosslinking   Karen Kroeger
• DNA isolation from hair   Gary Kucera
• Software package for SUNOS workstation   Rork D. Kuick
• Preparation of nuclei   Rajesh Kumar
• LINE inserts.   John Ladasky
• "RediLoad" PCR loading buffer recipe   John Ladasky
• Yeast Disruptants??   Michael Lassner
• Degenerate Primers in PCR   Michael Lassner
• His-tagged protein, urea & antibody production   Glenda Lawrence
• Database of the Korean biological journals (Abstracts)   Min-Ho Lee
• detection of protein (20-30kd) by western   Sam Lee
• His-Tag antibodies   Klaus Lehnert
• Subcloning 5kb insert   Leslie
• who makes disposable cuvettes for measuring DNA concentration?   Michael Levin
• nucleolus specific antibodies   Yanzhuang Li
• Removing Bacteria from yeast cultures   Michael Lichten
• Dam methylation and BclI   Michael Lichten
• Bcl digestion   Michael Lichten
• degenerate primers for pcr   J. Casey Lippmeier
• removal of hexahistidine tag by peptidase   Georg Lipps
• Fluorescent substrate for Peroxidase exc. 365 nm em. 445 nm?   Jerry Litt
• mutagenesis strategy   Lolis, Elias
• Advice on oligo probes for non-isotopic in situ hyb   Loren Joseph, MD
• Problems with Boehringers' DIG???   S. MORI
• Simple Method to Determine the Action Mode of a New Drug NEEDED!!!   lab of MYXO
• EDTA/Heparin & DNA stability   David MacHugh
• fuzzy bands on long seq gel?   David MacHugh
• colony hybridisations (DIG)   John F. Mackay
• Anti-DIG magnetic beads   Frank Maiwald
• Problems with Boehringers' DIG???   Frank Maiwald
• removing pigments   Alexandra Manevski
• protein assay in LDAO   Darren Mark Manley
• curious ligation question...   Ned Mantei
• curious ligation question...   Ned Mantei
• Problems with S1 digestion   Ned Mantei
• CLONTECH Cap Finder   Edoardo "Dado" Marcora
• Need seed DNA extraction protocol   S K Marquardt
• 1-Step MMLV-RT-PCR Buffer/Protocol?   S K Marquardt
• polypeptide separation   H M Marriott
• scanarray   Todd Martinsky
• How much blood do you use for DNA extraction?   Max
• Antibody for a nuclear marker?   Carl Mayers
• normalizing northerns of plants   Bill McKendree
• DNA concentration determination   Patrick McMillen
• Commercial source for SV40 large T-ag?   David Mead
• SeAP as a reporter gene   Jose E. Mejia
• DNAse I hypersensitivity protocol   Dr. J. Miano
• storage of bacterial plates   Sam Michaelson
• What is wrong with Genbank submissions!   Phillip San Miguel
• Sequencing redundancy   Phillip San Miguel
• RAW264.7 cells   Catherine Miller
• (none)   Catherine Miller
• protein solubilizing out of SDS-PAGE gel   Jay Miller
• Spooling coli DNA   Steve Minchin
• DNA quantitation : what a pain   Steve Minchin
• 5-Methyl dNTP Source?   John Mitchell
• his-tag   Souren Mkrtchian
• molbio-methods   Monalinas
• Inoue help - again...   Michael J. Morales
• "Mortal" RNAse Needed   Michael J. Morales
• How to prevent S35 contamination in tissue culture?   Stephen Morris
• nucleoside analogues: pH/conc.to dissolve??   Heidi Moss
• nucleoside analogues: pH/conc.to dissolve??   Heidi Moss
• nucleoside analogues: pH/conc.to dissolve??   Heidi Moss
• Help with synthesis of long sDNAs   Erik Mottl
• Hepl with synthesis of long cDNAs   Erik Mottl
• Removal of lipids from cell lysate?   Kevin Mulcahy
• Experience with Renilla Luciferase?   Fern E. Murdoch
• Help: Modified Western.   Fern E. Murdoch
• nucleotide analysis   Roger Murphy
• Solubilize peptide   Roger Murphy
• Dam methylation and BclI   Per Mygind
• Homemade ECL Plus reagents?   Norio Nakano
• Site-directed mutagenesis   Name
• HIS tagged protein from baculo   Darren A. Natale
• "RediLoad" PCR loading buffer recipe   David Neece
• Vectors for assaying splicing efficiencies (LacZ or luciferased based)   Nick
• Advice on sterilizing transfer pipets   Joe Nomen
• BNDC, MS-adenosylmethionine, and Genomic Mismatch Scanning   Kristen Nowak
• Anti-DIG magnetic beads   William O'Connell
• Anti-DIG magnetic beads   William O'Connell
• Anti-DIG magnetic beads   William O'Connell
• Pichia pains   Michael O'Donohue
• Cheap Electroporation?   Michael O'Donohue
• Lymphocytes   ERNESTO OVIDEO ORTA
• Help!! Signal Transduction Systems   ERNESTO OVIDEO ORTA
• Problems with Boehringers' DIG???   Chris Odt
• Northerns using bacterial RNA   Chris Odt
• histidine aromatic?   Martin Offterdinger
• [q]digitonin   Martin Offterdinger
• protein assay   Martin Offterdinger
• Long distance (RT) PCR   Martin Offterdinger
• TNP-cellulose   KwangChul Oh
• RNA extraction from blood   Dr GC Oliveira
• b-gal activity   Stephane Ory
• stripping RNA probes   Otto
• Reovirus - RNA extraction   P.C.
• Gel Drying   Laurent POULOUIN
• test   Anthony Page
• test   Anthony Page
• big DNA in XL-1 cells   Dr Dave Parcej
• Boyden Chamber-chemotaxis assay   Charlie Partridge
• polypeptide separation   R Patel
• polypeptide separation   R Patel
• Trans Golgi or Late Endosome marker   D. Pearton
• RNA: 260/280 reading   Karen Peck-Miller
• protein solubilizing out of SDS-PAGE gel   Peter Pediaditakis
• Lipid removal pre-SDS-PAGE   Peter Pediaditakis
• Acrylamide Polymerization   Peter Pediaditakis
• [Fwd: Fwd: gershon@hermes.law.stetson]   Ivor Percent
• What is the pI for IgG?   Pete
• 15-mer binding to membrane. Is it possible?   Arthur Osterop Ph.D.
• RNase Protection Assay; non-specific?   Arthur Osterop Ph.D.
• Gamma tubulin   David Phelan
• Killer gene/protein?   Markus Piotrowski
• Bcl digestion   Markus Piotrowski
• Antibody Purification: better method than western technique?   Markus Piotrowski
• Seamless cloning   Jan van der Ploeg
• Prokaryotic terminator detection from sequence Does anybody have a reliable method for detecting transcriptional   Justin Powell
• Detection of Prokaryotic terminators   Justin Powell
• PCR Jump Station   Horizon Scientific Press
• How to pull-out a specific PCR product?   Kiley R. Prilliman
• Solubilize peptide   Kiley R. Prilliman
• address for ExGen 500   David Pritchard
• SSCP kits   Dr.Narayana R.Isola
• Sodium free DNA   Dr.Narayana R.Isola
• tracking dye for cellulose acetate electrophoresis ?   RB
• Unsilanizing sequencing plates?   REWHLAB
• Freet Trip To Switzerland for Miptec ?   RManns7
• Boyden Chamber-chemotaxis assay   RManns7
• Hoefer TKO100 fluorometer   Rachel
• Agrobacterium strains   Ramanujam Raman/BCC265/384.2220
• Transformation of large fragments   Ramanujam Raman/BCC265/384.2220
• Kanamycin selection   Keith Rand
• jurkat transfections   Prakash K. Rao
• detection of protein (20-30kd) by western   Nicole Rapior
• His-Tag antibodies   Soren K. Rasmussen
• cloning large PCR bits   Dr. R. Ratcliff
• EDTA/Heparin & DNA stability   Achim Recktenwald
• High pH buffer?   Achim Recktenwald
• P1 plasmid kit   Rentia
• Michigan Cancer Foundation   Rentia
• Bio-Rad's gene gun tubing coating   Miranda v/d Rhee
• mobility vs. charge:mass ratio?   Phoebe Rice
• PS Software Joins Collaborative Electronic Notebook Systems Association's CENS Consortium as Vendor Member   Rich Lysakowski, Ph.D.
• PS Software Joins Collaborative Electronic Notebook Systems Association's CENS Consortium as Vendor Member   Rich Lysakowski, Ph.D.
• DNA concentration   Rick00100
• Genomic bacterial DNA PCR.   Mr SCG Rison
• Genomic bacterial DNA PCR [Please re-mail answers]   Mr SCG Rison
• sequence for pBAG   Stuart Rison
• Better chelator of zinc than EDTA?   Robot-Boy
• inoue competent cells help !   Robot-Boy
• Inoue help - again...   Robot-Boy
• curious ligation question...   Robot-Boy
• Separation of tiny from large fragments using spin columns   Don Rollins
• Address for Micronic   Jose Diaz Romero
• Help-Request: Unspecific Signal by Sekundary AB in Western Blot   Michael Rosemann
• Problems with Boehringers' DIG???   Michael Rosemann
• Database of transgenic mice?   Michael Rosemann
• Gamma tubulin   Steffen Roth
• Unsilanizing sequencing plates?   Hiranya Roychowdhury
• fuzzy bands on long seq gel?   Hiranya Roychowdhury
• his-tag   Hiranya Roychowdhury
• DNA concentration   Hiranya Roychowdhury
• Bcl digestion   Hiranya Roychowdhury
• Coorection Re: Bcl digestion   Hiranya Roychowdhury
• cDNA precipitation   George Rutherford
• Kanamycin selection   SHENGYANG
• shelf life of dNTPs at -20   SHENGYANG
• protein assay   Farida Safadi-Chamberlain
• Degenerate Primers in PCR   Tony Sanchez
• Contaminated -Taq   Tony Sanchez
• polyT primer for PCR   Michael Schaefer
• literature for rna secondary structure?   Heinz J. Schaefers
• Q: differentiating and evaluating different GFPs with fl   Wolfgang Schechinger
• Simple Method to Determine the Action Mode of a New Drug NEE   Wolfgang Schechinger
• PCR with degenerate primer   Wolfgang Schechinger
• LINE inserts.   Wolfgang Schechinger
• Q: on 293 cell and PDGF (-Receptor)   Wolfgang Schechinger
• Site-directed mutagenesis   Wolfgang Schechinger
• High molecular weight marker   Wolfgang Schechinger
• contaminated Taq   Wolfgang Schechinger
• Removing Bacteria from yeast cultures   Wolfgang Schechinger
• How do you calculate the molecular weight of the protein usi   Wolfgang Schechinger
• What is wrong with Genbank submissions!   Wolfgang Schechinger
• Q: Detecting farnesylation   Wolfgang Schechinger
• detaching/removing streptavidin from streptavidin coated ELI   Wolfgang Schechinger
• Ubiquitous primers   Wolfgang Schechinger
• Ciprofoxacin   Klaus A. Schmidt
• Help! Incredible high immunfluorescence background!   Thorsten Schmidt
• Fluorescent substrate for Peroxidase exc. 365 nm em. 445 nm?   Joerg Schmieding
• Database of transgenic mice?   John Richard Seavitt
• DTT and BME   John Richard Seavitt
• looking for a phage display cDNA library   Thomas Seib
• His-tagged protein purification: 96 well plate   Thomas Seib
• Help-Request: Unspecific Signal by Sekundary AB in Western Blot   Thomas Seib
• help   Serg
• In vitro transcription & translation   Todd Serulneck
• Gel Drying   Todd Serulneck
• How to prevent S35 contamination in tissue culture?   Sunil Shaw
• Northern blot re-probing   Sunil Shaw
• big DNA in XL-1 cells   Yelena Shifman
• "Mortal" RNAse Needed   Nathan Siemers
• Plate assays for B-lactamase   Nathan Siemers
• Help:Schistosoma japonicum GST   Juthamas Siripanee
• streptavidin columns?   Daniel Z Skinner
• Heteroduplex analysis, Fragile X by PCR   Heyko Skladny
• His-tagged protein, urea & antibody production   Koen De Smet
• His-tagged protein, urea & antibody production   Koen De Smet
• curious ligation question...   Koen De Smet
• Contaminated -Taq   Koen De Smet
• Genomic bacterial DNA PCR.   Koen De Smet
• HygB resistance of E.coli   Koen De Smet
• mutagenesis strategy   Harold E Smith
• High pH buffer?   Harold E Smith
• Commercially available cut vectors   Harold E Smith
• screening phage library   Ken Soderstrom
• GST fusion protein cleavage problem   Miss. L Somerville
• GelBond   Mark Spanevello
• EtBr and heat denatured DNA   Mark Spanevello
• Web Promo Power for WebMasters   Web Promotion Spider
• Q:Pichia kit   Dom Spinella
• What is the pI for IgG?   Dom Spinella
• MC1061/P3??   Dom Spinella
• kozak's sequence   Dom Spinella
• kozak's sequence   Dom Spinella
• Good general bioinformatics reference   Dom Spinella
• Ciprofoxacin   Dom Spinella
• semi-specific PCR   Dom Spinella
• semi-specific PCR   Dom Spinella
• Hepl with synthesis of long cDNAs   Dom Spinella
• [Fwd: Fwd: gershon@hermes.law.stetson]   Dom Spinella
• Antibody Purification: better method than western technique?   Dom Spinella
• Band-shift assay   Karen Spink
• Anti-H6 Ab for "pET" h6 tags   Bob Steinberg
• [Q] HhaI vs CfoI, HpaII vs HapII   Bob Steinberg
• RNase Protection Assay; DEPC for everything?   Siegfried Steinhauser
• Subcloning 5kb insert   Steve
• PAGE question   Steven Sullivan
• detection of protein   Clemens Suter-Crazzolara
• stable transfections   Clemens Suter-Crazzolara
• His-tagged protein, urea & antibody production   Vladimir Svetlov
• His-tagged protein, urea & antibody production   Vladimir Svetlov
• kozak's sequence   Vladimir Svetlov
• primer extention on on ABI 373 ?   Moez TORKI
• Does anybody know how to measure EEO?   Jichang Tan
• CAT 3MS and pSP72   Brian J. Taylor
• What is the pI for IgG?   Brian S. Thoma
• protein solubilizing out of SDS-PAGE gel   Peeter Toomik
• GETTING GUS (uidA) DNA AND PROTEIN SEQUENCES   Jorge Hernandez Torres
• resolution of DNA electrophoresis   TvdB
• Small protein Western Analysis   Darren Tyson
• Experience with Renilla Luciferase?   Darren Tyson
• interspecies hybridization   Developmental Biology Unit
• GelBond   Genetic Analysis Facility James Cook University
• Rapid Hyb   User
• green fluorescent protein redox ?   Michael Vagell
• shelf life of dNTPs at -20 ° C   Michael Vagell
• FISH problem   F. Vaillant
• Mouse 129 library   Robin Varghese
• CREB AB   Vidar
• purifying transkipted RNA for RT-PCR   Vidar
• water bears (tardigrades)   Vidar
• NP-40   Vita
• normalizing northerns of plants   Andreas Vogel
• RNA from plant roots   Andreas Vogel
• baculovirus kit opinions needed   VoiD
• Software package for SUNOS workstation   WZhao68
• Pichia pains   Beth Wapelhorst
• Have you seen these tip boxes??   Beth Wapelhorst
• DNA quantitation : what a pain   Beth Wapelhorst
• RNA extractions help!   Andrew Warkman
• Buy bio chemicals from manufacturer   Eddie Watkins
• Information about Thermojet Thermocycler   John Webb
• Plate assays for B-lactamase   Tom Webster
• pBI101 vectors   Ralf Weigel
• beta-actin   Dr. Cora Weigert
• sequencing   January Weiner
• Problems with S1 digestion   January Weiner
• Problems with S1 digestion   January Weiner
• Problems with S1 digestion   January Weiner
• resolution of DNA electrophoresis   January Weiner
• How to extract DNA from polyacrylamide gel   January Weiner
• RNA/PAGE electroblot   January Weiner
• Throw gloves after handling EtBr?   January Weiner
• EtBr and heat denatured DNA   January Weiner
• PAGE question   January Weiner
• >Sodium free DNA   Mr. T. Weissensteiner
• NEB T7 DNA polymerase for DNA sequencing?   Malcolm White
• Hoefer TKO100 fluorometer   C.S. Wilding
• literature for rna secondary structure?   C.S. Wilding
• histidine aromatic?   Randall C Willis
• Polypropylene Racks for Eppendorf tubes   Randall C Willis
• Kanamycin selection   Randall C Willis
• ? Ethidium Bromide Protection?   Randall C Willis
• big DNA in XL-1 cells   Randall C Willis
• problems with reverse primer   Mag. Dorian Winter
• Mobility shift problem...   John G. Wise
• Degenerate Primers in PCR   Harry Witchel
• Database of transgenic mice?   Harry Witchel
• DIG-labelled Actin-DNA   Anja Witke
• Radio-PAGE with [35S]-methionine labelled proteins   Torsten Witstruck
• Polypropylene Racks for Eppendorf tubes   Zhencai Wu
• cDNA Marathon Ready Kit by CLONTECH   Mark Wylenzek
• UPDATE: Bioinformation Search Center 4.0   Mousheng Xu
• !!! HELP-SURVEY-BONUS: BSC 4.0 !!!   Mousheng Xu
• UPDATE: Bioinformation Search Center 5.0!!!   Mousheng Xu
• Bcl digestion   Xiao-Hua Xue
• Plant transient expression   Ho Chul Yi
• Lyticase stock buffer   Yokosawa, Jonny
• biotinylated cloning   Yvonne.Rombout at rivm.nl
• histidine aromatic?   Z.Huang
• What is wrong with Genbank submissions!   Z.Huang
• rat genomic library   Jun Zhang
• Mouse Library   Jun Zhang
• RNA: 260/280 reading   Jun Zhang
• 1-Step MMLV-RT-PCR Buffer/Protocol?   Jun Mark Zhang
• Long distance (RT) PCR   Jun Mark Zhang
• Help! Incredible high immunfluorescence background!   Marieke R. Koedood Zhao
• problems with reverse primer   Tiejun Zhao
• Genetically Effective Cell Number (GECN)   X. Y. Zheng
• Genetically Effective Cell Number (GECN)   X. Y. Zheng
• Bcl digestion   X. Y. Zheng
• protocol for JM109 competent cell   Dr. Zhao Zhuoshen
• How to pull-out a specific PCR product?   aDamn
• Degenerate Primers in PCR   aDamn
• biotinylated cloning   aDamn
• curious ligation question...   aDamn
• LINE inserts.   aDamn
• Throw gloves after handling EtBr?   anonymous
• Throw gloves after handling EtBr?   anonymous
• Contaminated -Taq   bbeitzel at biomail.ucsd.edu
• Hot Start Wax Beads   bbeitzel at biomail.ucsd.edu
• Ciprofoxacin   bbeitzel at biomail.ucsd.edu
• semi-specific PCR   bbeitzel at biomail.ucsd.edu
• Site-directed mutagenesis   bpaeng at pado.krict.re.kr
• Tru9 I vs Tru 509 I enzyme recog. site?   bpaeng at pado.krict.re.kr
• Tru9 I vs Tru 509 I enzyme recog. site?   bpaeng at pado.krict.re.kr
• PCR Jump Station   bradtpublications at USA.NET
• packaging protocol   brett
• Contaminated -Taq   brett
• contaminated Taq   brett
• Hot Start Wax Beads   brett
• problems with reverse primer   brett
• Lipid removal pre-SDS-PAGE   Horticultural research center
• Oxygene entry into cells   che
• Interaction between Protein and DNA   chenln at PUBLIC.BTA.NET.CN
• Photomicrography Course   ciccio
• Transformation of E.Coli bugs   cocciac at rockvax.rockefeller.edu
• protein solubilizing out of SDS-PAGE gel   bipin k. dalmia
• packaging protocol   deborah_hailstones_at_emai at smtpgwy.agric.nsw.gov.au
• fuzzy bands on long seq gel?   deborah_hailstones_at_emai at smtpgwy.agric.nsw.gov.au
• in situ newsgroup?   deborah_hailstones_at_emai at smtpgwy.agric.nsw.gov.au
• EtBr and heat denatured DNA   deborah_hailstones_at_emai at smtpgwy.agric.nsw.gov.au
• Bacterial toxins   delios97 at hotmail.com
• how to use Hygromycin B   delios97 at hotmail.com
• Your web site...   e-bizness at e-bizness.com
• BioRad Gene Cycler   estephen at biology.as.ua.edu
• specs   doug feinstein
• Centrifuge   doug feinstein
• DNA silver stainning   garciai at uv.es
• Best transfection method for CHO cells   paul gold
• keep stable transfections   gzzhjr
• how to keep stable transfections?   gzzhjr
• how to use Hygromycin B   gzzhjr
• determine the apoptosis   gzzhjr
• request reagent   gzzhjr
• Rat Actin Probe   han
• genomic southern help   han
• genomic southern: help needed   han
• genomic southern: help needed   han
• genomic southern: help needed   han
• genomic southern: help needed   han
• New life science reagents resource   corniche information
• Northern blot re-probing   is.bbsrc.ac.uk
• PFGE/Southers   jdc6 at psu.edu
• RNA extractions help!   joanna
• ADV: checks by fax   juliek at ROCKETMAIL.COM
• ADV: checks by fax   juliek at ROCKETMAIL.COM
• DNA concentration   paul lacy
• PCR with degenerate primer   qin ling
• His-tagged protein purification: 96 well plate   feng liu
• detaching/removing streptavidin from streptavidin coated ELISA plate?   m.fennema
• Pamela Anderson Lee Sex Video Tape   menssen
• phosphatidylinositol synthase   mjohnson at biology.as.ua.edu
• PCR Jump Station   orders at USA.NET
• Pamela Anderson Lee Sex Video Tape   ownacopy at POBOX.COM
• What is the highest DNA concentration in a buffer   peng.36 at postbox.acs.ohio-state.edu
• PCR machines   bill pohajdak
• Earn $450 Commissions Part-time!   profits1998 at POBOX.COM
• DNA and protein analysis and fingerprinting of hybrids   quique at netsys.hn
• stable transfections   ronlab
• stable transfections   ronlab
• Commercially available cut vectors   ronlab
• PinPoint expression system   ruben.varela-calvino at kcl.ac.uk
• FHIT Ab available?   s.pritchard
• RYNO Seminar -- 18 February   sbcom at WAINFLEET.IDS.ON.CA
• RNA from plant roots   schmitz
• ***IT REALLY WORKS***YOU MUST READ THIS!!!   smd
• ZAP-Express excision problem   smoothies
• register   yang tao
• RNase Protection Assay; DEPC for everything?   theodorn at medlib.georgetown.edu
• help: cDNA to Genetic Organization   tjc at itsa.ucsf.edu
• ECACC   un691cs at genius.embnet.dkfz-heidelberg.de
• HIS tagged protein from baculo   un691cs at genius.embnet.dkfz-heidelberg.de
• stable transfections   un691cs at genius.embnet.dkfz-heidelberg.de
• Contaminated -Taq   un691cs at genius.embnet.dkfz-heidelberg.de
• antibody to c-RET (proto-oncogen product of c-ret)   un691tl at genius.embnet.dkfz-heidelberg.de
• test   xiaolin wu
• High molecular weight marker   wulf-dirk.leuschner at tuebingen.mpg.de
• [Q] HhaI vs CfoI, HpaII vs HapII   yshan at tiamat.kaist.ac.kr
• unsubscribe   zhangyl at PUBLIC.CC.JL.CN
• unsubscribe   zhangyl at PUBLIC.CC.JL.CN

Last message date: Sat Feb 28 23:02:07 EST 1998
Archived on: Sun Mar 6 21:41:24 EST 2005

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