FasL mono, di-, and trimers

John Richburg John_Richburg at mail.utexas.edu
Tue Jan 6 14:23:22 EST 1998

I am doing Western analysis of FasL, specifically for the soluble form
using an antibody that recognizes the proposed cleaved and released
portion of the FasL protein. I homogenized the tissue in buffer that
contains 1 %NP40, 0.5% sodium deoxycholate, and 0.1% SDS plus PMSF,
aprotinin and sodium orthovanadate.

My question is, could dimers or trimers of this protein exist under these
detergent conditions? I seem to see several high mw bands but am having a
hard time justifying them. I even see these band in the positive control
sample of purified soluble FasL protein.

Has anyone had a similar experience? Can noncovalently bonded proteins
remain together under the conditions above?

Thanks in advance,


John Richburg
Assistant Professor
College of Pharmacy
Division of Pharmacology and Toxicology
The University of Texas at Austin

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