non-radioactive detection kit

Bill McKendree mckendre at ASRR.ARSUSDA.GOV
Tue Jan 6 08:49:59 EST 1998


Dan,

I prefer biotin labeling, others perfer DIG, both of which are 
commercially available from NEB and Boehringer respectively.  
Both systems have good sensitivity when the probe is prepared well, and 
precautions are taken to minimize background.

Bill McKendree
USDA-ARS
Orlando, FL
(407) 897-7335 phone



On 6 Jan 1998, Dan Pelah wrote:

> I am looking for chemiluminescent detection kit for southern and northern
> blot analysis.
> Does any one has any experience using such a kit, and from your knowledge
> which kit has the best performance?
> 
> Dan Pelah
> 
> 
> 
> 
> 
> 



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