adj8n at virginia.edu
Tue Jan 6 08:30:10 EST 1998
Does anyone know of a way to irreversibly inhibit B-gal reporter? I want
to look at transcription rates for a low activity, long half-life gene
product. Looking at B-gal overall only tells me what cumulative expression
is. I want to look at the expression at specific time points. Here is the
clincher - I have to do the analysis in aortas of whole animals. There is
too much matrix around, so I cannot do nuclear run-on with aortic tissue
nuclei. So the trick may be to poison existing B-gal, then look at
reporter activity of newly synthesized material only.
Thanks in advance.
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