S1 RNA Analysis

January Weiner jweiner1 at ix.urz.uni-heidelberg.de
Wed Jan 7 06:34:39 EST 1998

Hello there,
	I'm looking for a decent protocol of S1 RNA 3' end mapping. I have
the one of Maniatis, of course, but it is quite old. Has anyone here
experience with this method? I'd like to digest DNA/RNA ss in DNA/RNA
hybrids. The 32-P cDNA used for hybridisation should be ds. 
	Thank you,
There is a certain impertinence in allowing oneself to be burned for an
opinion.                          -- Anatole France

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