Easiest Method of Synchronizing CHO Cells

Martin Offterdinger a8803349 at unet.univie.ac.at
Thu Jan 8 03:21:58 EST 1998


On 7 Jan 1998 07:43:42 -0800, paulgold at MVP.NET (paul gold) wrote:

>Dear Netters,
>
>I've never synchronized a population of cells.  I've looked up a few
>protocols and the most prevalent one I see is synchronization using
>hydroxyurea.  Is this the most widely used, easiest and most efficient
>method?  Regards.
>
>Paul Gold
There is also a method which involves Thymin in the a culture medium
(leads to an S-phase arrest) followed by nocodazole treatment
(inhibits microtubule formation). The mitotic cells are finally
harvested by shaking them off.
Martin




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