Easiest Method of Synchronizing CHO Cells

[Martin Offterdinger]

On 7 Jan 1998 07:43:42 -0800, paulgold at MVP.NET (paul gold) wrote:

>Dear Netters,
>
>I've never synchronized a population of cells.  I've looked up a few
>protocols and the most prevalent one I see is synchronization using
>hydroxyurea.  Is this the most widely used, easiest and most efficient
>method?  Regards.
>
>Paul Gold
There is also a method which involves Thymin in the a culture medium
(leads to an S-phase arrest) followed by nocodazole treatment
(inhibits microtubule formation). The mitotic cells are finally
harvested by shaking them off.
Martin