T7 RNA polymerase phage?

Peter Wang plw at mrc-lmb.cam.ac.uk
Thu Jan 8 12:23:45 EST 1998


Brad Pietz wrote:
> I am attempting to express a phage protein in E. coli that appears to be
> quite toxic.  I am able to transform JM109 just fine with my pET vector
> construct but am unable to transform any strain containing T7 RNA
> polymerase (BL21 DE3).  I have heard of people infecting JM109 with
> recombinant phage containing T7 polymerase.  Does anyone know of a source
> for these phage (lambda or M13)?

Invitrogen sells M13/T7 phage as part of their pRSET expression kit; I'm 
not sure if it's available separately - you could check with them.
This question was asked before a while back, and I gave this same 
answer, so sorry for the a repetition.  

Cheers,
- Peter

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Peter Wang, M.D., Ph.D.
MRC Centre for Protein Engineering,
Hills Road, Cambridge, CB2 2QH, England

Tel (01223) 402104  (international calls +44-1223-402104)
Fax (01223) 402140  (     "          "   +44-1223-402140)
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