DD-RT-PCR

Brian btreacy at afns.ualberta.ca
Thu Jan 8 13:48:32 EST 1998


  Hi everyone!

  Just about to attempt my first DD-RT-PCR! I'm thinking of using the MMLV
reverse transcriptase and amplitaq as recommended by GenHunter. I was
wondering if the use of alternate enzymes such as RT Expand and Amplitaq
gold or other such as the Titan mix (Taq and Pwo DNA polymerases) would
provide better results. Any recommendations would  be greatly appreciated!

  Thanks in Advance,


   Brian





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