Glycerol and Tm

vilimf01 at mcrcr6.med.nyu.edu vilimf01 at mcrcr6.med.nyu.edu
Wed Jan 7 10:32:25 EST 1998


Hi All,

   Does anyone know how glycerol functions in PCR?  Does it stabilize
the polymerase, or destabilize base pairing like formamide and DMSO. 
If it does destabilize, how does it compare to DMSO and formamide?  Is
there any way to calculate the Tm for a particular percentage of
glycerol the way one does for formamide?  TIA

					Best Regards,  SVEN



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