No plasmid from trasformed BL21(DE3)
khuang at BIOC.RICE.EDU
Fri Jan 9 19:11:10 EST 1998
Thanks your guy's suggestion. Let me ask you more about BL21(DE3).
I am sure (100%) BL21(DE3) is competent(I make electro-competent cells).
I usually transform my ligation into BL21(DE3) and then pick up a few
colonies to do miniprep and check the expression, I can save at least two
days for not transforming ligation into other cells like XL1, JM109 at
first. It really worked for me before (at least I could success from two of
three clonies), but recently, I was not so luck (one of five or zero success),
you guy say, BL21(DE3) is difficult, Why?
How do you do minprep from BL21(DE3)?
More information about the Methods