No plasmid from trasformed BL21(DE3)

Ke-Xue Huang khuang at BIOC.RICE.EDU
Fri Jan 9 19:11:10 EST 1998

Hi, Netters,

Thanks your guy's suggestion. Let me ask you more about BL21(DE3).
I am sure (100%) BL21(DE3) is competent(I make electro-competent cells).

I usually transform my ligation into BL21(DE3) and then pick up a few 
colonies to do miniprep and check the expression, I can save at least two 
days for not transforming ligation into other cells like XL1, JM109 at 
first. It really worked for me before (at least I could success from two of 
three clonies), but recently, I was not so luck (one of five or zero success), 
you guy say, BL21(DE3) is difficult, Why?

How do you do minprep from BL21(DE3)?


More information about the Methods mailing list