DD-RT-PCR

[D.L.Clarke]

In article <v02130500b0dac1575df9@[129.128.131.32]>,
btreacy at afns.ualberta.ca (Brian) wrote:

>   Hi everyone!
> 
>   Just about to attempt my first DD-RT-PCR! I'm thinking of using the MMLV
> reverse transcriptase and amplitaq as recommended by GenHunter. I was
> wondering if the use of alternate enzymes such as RT Expand and Amplitaq
> gold or other such as the Titan mix (Taq and Pwo DNA polymerases) would
> provide better results. Any recommendations would  be greatly appreciated!
> 
>   Thanks in Advance,
> 
> 
>    Brian



Dear Brian,

I have just performed my first ddrtpcr experiment using the RNAimage kit
from GenHunter. And it gave me moderate success, as I could identify
several putative bands for further investigation.  I used the supplied
MMLV reverse transcriptase and was advised to use the  Qiagen Taq
polymerase by GenHunter.  The only advantage I could see was the cost, as
Qiagen Taq is $70 cheaper than  AmpliTaq.


Hope this helps a little bit


Dave Clarke 

dlc22 at cornell.edu

-- 
Dave Clarke
Department of Plant Pathology
Cornell University
NYSAES
Geneva, NY. 14456

Phone 315-787-2332
Fax   315-787-2389

e-mail dlc22 at cornell.edu