IEF

[Robert Woodward]

Dear All,

Does anyone in the Cambridge (UK) area have any experience with IEF?
I would like to know the IEP of a pertially purified protein that I
have antibodies to. I only have standard 1D miniprotean II equipment
and I first of all need to know if this could be used in some way, and
secondly can you western blot IEF gels and then probe with antibodies so
as to identify my protein of interest?  If you need specialist equipment
is anyone prepared to run a couple of samples out for me (or loan me some
equipment)? obviously I would be prepared to pay for materials used etc..

Thanks very much

Robert Woodward

Email rw200 at cus.cam.ac.uk
Cambridge University
Dept Obs+Gynae
Rosie Maternity Hospital
Cambridge