Melting temp formula for RNA:RNA

Andy Phillips andy.phillips at bbsrc.ac.uk
Tue Jan 13 06:27:54 EST 1998


I've started using riboprobes for my Northerns and I want to calculate
the melting temperature of RNA:RNA hybrids, so that I can use a
reasonably stringent wash temperature. I would normally wash my DNA:RNA
Northerns at 60-65C in 0.1xSSc/0.1% SDS. I have formulae for DNA:DNA and
DNA:RNA; can anyone point me to an equivalent formula for RNA:RNA?

Thanks.

Andy


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