Double insert ligation

Richard Grant rgrant at cmtech.co.uk
Tue Jan 13 05:54:11 EST 1998


Hi,

Blunt ends should be fine if everything else is sticky.  If you don't
dephosphorylate the blunt ends it should work fine.

I would suggest however that you titrate the amounts of inserts (wrt
each other) in the ligation, keeping vector constant.  Makes for a big
expt but should work ;-)

Richard P. Grant DPhil | Cambridge Molecular Technologies
Senior R&D Scientist   | http://www.cmtech.co.uk/
Tel: +44 1223 508345   | 
Fax: +44 1223 506541   | 

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