help:clone a large fragment

Christophe Jacob jacob at
Tue Jan 13 11:21:36 EST 1998

In article (Dans l'article) <34B80E3C.2A3 at>,
ls13 at ZSULINK.ZSU.EDU.CN (wei yongjie) wrote (écrivait) :

> Hello,bionet users
>    I have tried to clone a fragment larger than 20kbp with a plasmid 
> vector,but each time I failed?Is it possible  to clone such a large 
> fragment with a plasmid vector?Please give me some advice on it.

I think it is necessarly to have very special plasmid to clone so long
fragments. Cosmids are the best for me.

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