Help!!>> DNA Replacement
vurk at hymail.hanyang.ac.kr
Tue Jan 13 21:39:30 EST 1998
I'd like to Replace DNA between
Plasmid DNA and Bacterial Genomic DNA.
This Replacemnt of the DNA is work for
all the General plasmid and Host?.
I use Plasmid 'pT7T3(pUC19 derivate)', and Host as a 'Moraxealla'
and They Both have a Homologous Region.
After Replacemention, I must remove all the plasmid in Host(Moraxella).
According to other person's thesis(from German),
they use SUICIDE plasmid vector to remove the plasmid.
But Is it OK if I treat "Plasmid Curing' Instead ?
I am Desperate for these Answers.
Please Answer Me Gently.
More information about the Methods