GEX-GST induction

Pierre Lindenbaum lindenb at jouy.inra.fr
Wed Jan 14 09:39:55 EST 1998


Hello,

I would like to purify a 'GST fused protein' from 50ml of an induced
culture. It worked the first time when I tried to induce 2m of culture
because I was able to see a big band on my gel. Since I want to purify a
large scale culture, my fused protein do not want to be expressed. 

I always start from the same frozen tube.
I use LB medium, Ampicillin.
I induce at Abs600=0.6 with IPTG 0.1M

	Could anybody help me ?

		thank you in advance
	
				PIERRE	L.

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