GST fusion protein solubility

Frederik Boernke boernke at ipk-gatersleben.de
Wed Jan 14 17:34:09 EST 1998


Hi Koen,

did you ever try induction at lower temps, i.e. 28 or 15 degrees with
your pQE construct? It is often described to yield more recombinant
protein in the soluble fraction. I am having the same problem and want
to know whether this is worth a try.

Ricky 

******************************************************************

Frederik Boernke
Research Group of  Molecular Plant Physiologie
Institute for Plant Genetics and Crop Plant Research (IPK)
Corrensstr. 3
06466 Gatersleben
Tel.  039482 -5 321
Fax. 039482 -5 515
e-mail: boernke at ipk-gatersleben.de
http://www.ipk-gatersleben.de



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