site directed mutagenesis

[Vladimir Svetlov]

In article <69h1v1$1hj at news.acns.nwu.edu>, ageorge at casbah.acns.nwu.edu wrote:

> -- Does anyone out there know what is the best kit to use for site directed 
> mutagenesis. Thanks in advance

Awarding the "best kit" title would require an impractical for any single
user/lab amount of redundant experimentation; however, from personal
experience I can recommend Stratagene's Quickchange and Promega's
pALTER/pSELECT kits. Both are reliable and easy to use with reproducibly
high yields.
Regards,
V.

-- 
Vladimir Svetlov
McArdle Lab for Cancer Research
Dept. Oncology
UW-Madison
1400 University Ave.
Madison, WI 53706