site directed mutagenesis

Vladimir Svetlov svetlov at
Wed Jan 14 12:57:54 EST 1998

In article <69h1v1$1hj at>, ageorge at wrote:

> -- Does anyone out there know what is the best kit to use for site directed 
> mutagenesis. Thanks in advance

Awarding the "best kit" title would require an impractical for any single
user/lab amount of redundant experimentation; however, from personal
experience I can recommend Stratagene's Quickchange and Promega's
pALTER/pSELECT kits. Both are reliable and easy to use with reproducibly
high yields.

Vladimir Svetlov
McArdle Lab for Cancer Research
Dept. Oncology
1400 University Ave.
Madison, WI 53706

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