GST fusion protein solubility

Koen De Smet k.desmet at nospam.ic.ac.uk
Thu Jan 15 10:27:33 EST 1998


Frederik Boernke wrote:
> 
> Hi Koen,
> 
> did you ever try induction at lower temps, i.e. 28 or 15 degrees with
> your pQE construct? It is often described to yield more recombinant
> protein in the soluble fraction. I am having the same problem and want
> to know whether this is worth a try.
> 
I tried it at room temperature for two of them, but id didn't make any 
difference.

Koen De Smet
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